Literature summary for 2.4.2.2 extracted from

Balaev, V.V.; Prokofev, I.I.; Gabdoulkhakov, A.G.; Betzel, C.; Lashkov, A.A.
Crystal structure of pyrimidine-nucleoside phosphorylase from Bacillus subtilis in complex with imidazole and sulfate (2018), Acta Crystallogr. Sect. F, 74, 193-197 .

Search for more literature for 2.4.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged enzyme	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme in complex with imidazole and sulfate, sitting drop vapour diffusion, mixing of 0.002 ml of 16 mg/ml protein in 20 mM Tris-HCl, 500 mM KCl with 0.002 ml of reservoir solution containing 0.2 M ammonium acetate, 0.1 M bis-Tris, pH 5.5, X-ray diffraction structure determination and analysis at 1.88 A resolution	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P39142	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinnat His-tagged enzyme by nickel affinity chromatography	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	pyrimidine-nucleoside phosphorylase catalyzes the phosphorolytic cleavage of thymidine and uridine with equal activity	Bacillus subtilis	?	-	-

Subunits

Subunits	Comment	Organism
homodimer	the enzyme has a two-domain structure and functions as a dimer	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
BsPyNP	-	Bacillus subtilis
PDP	-	Bacillus subtilis
PYNP	-	Bacillus subtilis
pyrimidine-nucleoside phosphorylase	-	Bacillus subtilis

General Information

General Information	Comment	Organism
evolution	pyrimidine-nucleoside phosphorylase is a member of the NP-II family, implying that it has a two-domain structure and functions as a dimer	Bacillus subtilis
additional information	analysis of the three-dimensional structures of the nucleoside-binding sites in subunits A and B of BsPyNP complexed with imidazole. The imidazole (IMD) molecule is localized in the active site of both subunits. IMD interacts with the enzyme residues via hydrogen bonds and a stacking interaction	Bacillus subtilis

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Release 2023.1 (January 2023)